Friday, December 15, 2017

Plz DONATE

KAMDHENU(Regd.Trust)
Registered in section 12(A) No.CIT-1/T/120(K-41)Agra/2004-05/3261
Donations for this trust are eligible for tax relief U/S-80G of income tax act 1961

KAMDHENUDHAM, PATNAGARH
Our organization (kamdhenu) is a national organization.  we try to develop the poor-villages in Odisha by training the farmers on cow based cultivation and cottage industry with preserving forest, water, soil and wild life. In Odisha more than 200 villages have already been undertaken and village committees have been formed. For this purpose 7 acres of land has been taken (2.5 acres from donation) 13 Kilometers away from Patnagarh city.

What we humbly need from you:-
1- Cow shed: 60’x20’ tin thatched shed. Costing about =Rs. 3,60,000/-
2- Office: 20’x15’ R C C roof Costing about= Rs.150,000/-
3- Medicine manufacturing shed: 10’x40’ hall Costing about =Rs. 2,00,000/-
4- Compost(manure) making pit(10.no.s) 6’x12’x6’ pit@=Rs 5000/- each =Rs.50,000/-
5- Shelter for old age & disable Cattle (100 nos.) 40 sq. ft/cow , total 4000 sq ft @Rs. 30/ sq. ft = Rs. 1,20,000/-
6- Feed go-down (dried fodder store)= Rs. 50,000/-
7- Free health centre: 15’x20’ costing about Rs:1,80,000/-
8- Residential quarter: 4-no.s x 1,00,000/- =Rs. 4,00,000/-
9- Borewell ,motor and over head tank with all fittings Rs. 1,20,000/-

Objectives of KAMDHENUDHAM, PATNAGARH
1- Farmer training about cow based cultivation.
2- Ayurvedic (panchgavya) medicine.
3- Bio-organic product.
4- Self employment scheme for rural people.
5- Free health service.
6- Cottage industry.
7- Preservation and improvement of live stock.
8- Herbal garden.

For details plz visit: www.sevika.org/, www.kamdhenudham.blogspot.com/
Email:info@sevika.org, kamdhenudham@gmail.com/ymail.com
Mobile: +91 9938712663


Donation for Old Age Home


Donation for Old Age Home
AmountDetails
INRUSD
100020Medicines Boxes 
2000401 Meal / Bed Side Lockers / Bed Sheets
300060Almirah / Cup Board
5000100SEMI (Fowler) / Tilt Bed / A Days food
10,000200Television / Air Cooler
15,000300  Water Cooler/ Kitchen Equipment / Kitchen Crockery
20,000400To look after an aged person per year
25,000500Dining Hall Furniture
50,0001000Dormitory Construction/Laundry Equipment / Freezer Box
1 Lakh2000Single Room in memory of your beloved ones./fixed deposit towards corpus fund.
1.5 Lakhs3000Double Room
2 Lakhs4000Medical Equipment / Physiotherapy Equipment
2.5 Lakhs5000Ambulance
3 Lakhs6000Sharing Room Unit
5 Lakhs10,000Construction Dormitory (20 Beds)

Donation for Cow Shelter

We are planning to construct “Shri KAMDHENU GAU SEVA KENDRA” with adequate facilities for Medical Treatment and housing for 100 cows.

Requirement for “Shri kamdhenu Gau Seva Kendra”
AmountDetails
Rs.2,51,000Compound wall with Gate
Rs.3,51,000Cow Shed Construction
Rs.50,000Ceiling Fans for Shed 50 nos. at Rs. 1000 each
Rs.1,51,000Water supply facilities with Tube-Well
Rs.2,50,000Grass Godown
Special Scheme for “Shri Kamdhenu Gau Seva Kendra”
AmountDetails
Rs.5000Operation and Medical expense for 1 cow
Rs.3501One day Fodder expense for 25 cows
Rs.11,001Kansar Seva for cows On Birthday / Gokulashthami/Ekadasi/Paryushan
Rs.2000Ambulance expenses for one cow one time
Life Membership for “Shri Kamdhenu Gau Seva Kendra” (From interest income)
AmountDetails
Rs.1,51,001Seva for 31 cows for 12 months
Rs.75,001Seva for 31 cows for 6 months
Rs.40,001Seva for 31 cows for 3months
Rs.10,000Seva for 31 cows for 1 months
Interests:
         Service to the Cow & mankind, research and developments, Health, Environment, Agriculture, Horticultur, Plantetion etc…….

Vision:
       We will create aditee's own Bio-organic world. 

About me:
          Every day, about 30 consultatio attend and accordingly give the respective Ayurvedic Diagnosis and Panchagavya (cow's medicinal products) Treatments. Here each and everyone may step in and receive the healing blessing of Go Mata, The Mother of Mankind, Goddess of Gods and the Worshiped Deity of Parameshvar, Shri Govinda.

I feel...:          
         (Kamdhenu Gosthhi) is creating a protective layer for resistance of future unexpected exposures. How can we protect ourselves and universe???? Assure profoundly study our safety programs.
Following promise I take to myself:
1- Every day I’ll protect at least a tree.
2- Every week I'll plant a new tree.
3- I’ll do havan (YAGNYA ) and pray to sun for genuine nature & avoid sun ’s warmness.

Our medicines:
Kamdhenu makoya ark
Kamdhenu yogendra rash
Kamdhenu trayodasang google
Kamdhenu rasonadi google
Kamdhenu medohar google
Kamdhenu abhaa google
Kamdhenu kachnar google
Kamdhenu keisor google
Kamdhenu asmarinasak churn
Kamdhenu abipatikar churn
Kamdhenu Ubtan
Kamdhenu soap
Kamdhenu iye drops
Kamdhenu malham
Kamdhenu mazik tail
Kamdhenu chyavanprass
Etc….



  1. Donations: Donations to kamdhenu dham vrindaban are tax exempt in India under Section 80 G of the Income Tax Act.1961.

Friday, March 27, 2015

kidney problem

Displaying 20150304_095413.jpg             this report is only 15 days taken panchagavya medicines after reduce critine 3,7 to 2.2

Sunday, July 27, 2014

Maha panchagavya ghreet

Maha Panchagavya Ghrita – Benefits, Dosage, How To Use, Side Effects, Ingredients, Reference

Maha Panchagavya Ghrita is an Ayurvedic medicine, in herbal ghee form.This medicine has ghee as its base. It is used for preparatory procedure for Panchakarma and also as medicine.
Please note that it is different from Panchagavya ghrita
Maha Panchagavya Ghrita benefits: 
It is used as medicine and also in preparatory procedure called snehakarma for the treatment of fever, epilepsy, fistula, inflammation, piles, liver diseases, anemia, cough and psychiatric conditions.
Synonyms: Maha Panchagavya ghritam, Maha Panchagavy Ghrit
Effect on Tridosha - Calms Vata, Pitta and Kapha.

Maha Panchagavya Ghrita dosage: 
As medicine – quarter to half teaspoon with water, usually before food, once or twice a day, or as directed by Ayurvedic doctor.
For Panchakarma preparation – Snehana procedure, the dose depends on the disease status and the judgement of Ayurvedic doctor.
Maha Panchagavya Ghrita Side Effects: 
There are no known side effects with this medicine. However it is best to use this product under medical supervision. Self medication with this medicine is discouraged.
People with diabetes, high cholesterol, heart diseases and high BP should excise precaution.
In very high dose, it may cause diarrhoea and indigestion.
Maha Panchagavya Ghrita Ingredients: 
96 grams of each of
Dashamoola group of roots -
Bilva – Aegle marmelos
Agnimantha – Premna mucronata
Shyonaka – Oroxylum indicum
Gambhari – Gmelina arborea
Patala – Stereospermum suaveolens
Shalaparni – Desmodium gangeticum
Prinshnaparni – Urarica picta
Gokshura – Tribulus terrestris
Brihati – Solanum indicum
Kantakari – Solanum xanthocarpum,
Triphala – Haritaki (Terminalia chebula), Vibhitaki (Terminalia bellirica) Amla (Emblica officinalis)
Haridra – Turmeric
Daruharidra – Berberis aristata
Kutaja – Holarrhena antidysenterica
Saptaparna – Alstonia scholaris
Apamarga – Achyranthes aspera
Nilini - Indigofera tinctoria
Katurohini – Picrorhiza kurroa
Shampaka – Cassia fistula
Pushkaramoola – Inula racemosa
Phalgumoola – Ficus carica
Duralabha – Alhagi psudalhagi
water for decoction – 12.288 liters, boiled and reduced to 3.072 liters
powders of 12 grams of each of
Bharngi – Clerodendron serratum
Patha – Cissampelos pariera
Adhaki – Cajanus cajan
Kumbha – Operculina turpethum
Nikumbha – Baliospermum montanum
Trikatu – pepper, long pepper and ginger
Rohisha –  Cymbopogon martinii
Murva - Marsdenia tenacissima
Bhutika - Trachyspermum ammi
Bhunimba – Andrographis paniculata
Shreyasi – Piper chaba
Sariva – Hemidesmus indicus
Madayantika – Lawsonia inermis
Agni – Plumbago zeylanica
Nichula - Barringtonia acutangula
Gomaya swarasa – the water extract prepared from cow dung – 768 ml
ksheera – cow milk - 768 ml
Dadhi – cow cheese - 768 ml
Mutra – Cow urine - 768 ml
Ghritham – ghee – 768 grams
The above combination is heated till herbal ghee is prepared.

PANCHAGAVYA

Benefits, Dosage, How To Use, Side Effects, Ingredients, Reference

Panchagavya Ghrita is an Ayurvedic medicine, in herbal ghee form.This medicine has ghee as its base. It is used for preparatory procedure for Panchakarma and also as medicine. Panchagavyam refers to five different components of cow products.
Panchagavya Ghrita benefits: 
It is widely used as medicine and also in preparatory procedure called snehakarma for the treatment of
neurological and psychiatric conditions, fever, liver diseases such as jaundice.
Effect on Tridosha - Calms Pitta and kapha

Panchagavya Ghrita dosage: 
As medicine – quarter to half teaspoon with water, usually before food, once or twice a day, or as directed by Ayurvedic doctor.
For Panchakarma preparation – Snehana procedure, the dose depends on the disease status and the judgement of Ayurvedic doctor.

It is usually administered with warm water.Ghrita Side Effects: 
There are no known side effects with this medicine. However it is best to use this product under medical supervision. Self medication with this medicine is discouraged.
People with diabetes, high cholesterol, heart diseases and high BP should excise precaution.
In very high dose, it may cause diarrhoea and indigestion.

Panchagavya Ghrita Ingredients: 
Gomaya swarasa – the water extract prepared from cow dung – 3.072 liters
ksheera – cow milk – 3.072 liters
Dadhi – cow cheese – 3.072 kg
Mutra – Cow urine – 3.072 liters
Ghritham – ghee – 768 grams
The above combination is heated till herbal ghee is prepared.
Ghrita Side Effects: 
There are no known side effects with this medicine. However it is best to use this product under medical supervision. Self medication with this medicine is discouraged.
People with diabetes, high cholesterol, heart diseases and high BP should excise precaution.
In very high dose, it may cause diarrhoea and indigestion.

Panchagavya Ghrita Ingredients: 
Gomaya swarasa – the water extract prepared from cow dung – 3.072 liters
ksheera – cow milk – 3.072 liters
Dadhi – cow cheese – 3.072 kg
Mutra – Cow urine – 3.072 liters
Ghritham – ghee – 768 grams
The above combination is heated till herbal ghee is prepared.
Ghrita Side Effects: 
There are no known side effects with this medicine. However it is best to use this product under medical supervision. Self medication with this medicine is discouraged.
People with diabetes, high cholesterol, heart diseases and high BP should excise precaution.
In very high dose, it may cause diarrhoea and indigestion.

Panchagavya Ghrita Ingredients: 
Gomaya swarasa – the water extract prepared from cow dung – 3.072 liters
ksheera – cow milk – 3.072 liters
Dadhi – cow cheese – 3.072 kg
Mutra – Cow urine – 3.072 liters
Ghritham – ghee – 768 grams
The above combination is heated till herbal ghee is prepared.

Sunday, November 6, 2011

Effects of Kamdhenu Ark


Effects of Kamdhenu Ark and Active Immunization by Gonadotropin
Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic 
Indices (GSI) and Sperm Parameters in Male Mus musculus
Javid Ahmad Ganaie, Varsha Gautam, Vinoy Kumar Shrivastava
*
- Endocrinology Laboratory, Department of Biosciences, Barkatullah University, Bhopal, India
Abstract
Background: Active immunization against GnRH decreases the secretion of gonadotropins and causes cessation of gonadal function, thereby, inducing infertility. Based
on the immunoenhancing activity of Kamdhenu ark (distilled cow urine), this study
was performed to evaluate its effects on the gonadosomatic indices (GSI) and sperm
parameters in male mice receiving a GnRH contraceptive vaccine. 
Methods: Sixty adult male mice of Parke’s strain were divided into three groups of
twenty. Group I served as the controls, while group II was immunized by GnRH-BSA
conjugate (50/0.2/35 µg/ml/g BW) by four intraperitoneal injections at different intervals on days 1, 30, 60 and 90. However, group III was supplemented daily by oral
Kamdhenu ark (100  ppm) along with GnRH-BSA immunizations. The animals were
sacrificed after 30, 60, 90 and 120 days and their testis and epididymis were dissected
out weighed and semen analysis was performed. 
Results: GSI values, sperm motility, sperm count and sperm morphology in male Mus
musculus were decreased significantly in all the experimental groups as compared to
the control group (p<0.01). Kamdhenu ark significantly enhanced the effect of GnRH
vaccine on the aforesaid parameters especially in 90 and 120 days treated groups
(p<0.05).
Conclusion: The changes witnessed in sperm parameters suggested that the GnRHBSA immunization suppressed the activities of gonadotropins and testosterone directly through hypothalamo-hypophysial-gonadal  axis and indirectly by acting on the
testes which may modulate the sperm morphology, sperm count and motility. However, Kamdhenu ark seems to have enhanced these effects because of its immunemodulatory properties too.
Keywords:  GnRH-BSA, Gonadosomatic indices (GSI), Immunization, Kamdhenu ark,  Mus
musculus, Sperm parameters.
To cite this article: Ganaie JA, Gautam V, Shrivastava  VK. Effects of Kamdhenu Ark and Active
Immunization by Gonadotropin Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic Indices
(GSI) and Sperm Parameters in Male Mus musculus. J Reprod Infertil. 2011;12(1):3-7.
Background
        onadotropin-releasing hormone (GnRH) con-         
        trols the production of gonadotropins, thereby having an orchestrating effect on the reproductive hormone cascade and spermatogenesis (1).
Active immunization against GnRH has successfully suppressed the secretion of gonadotropins
and decreased sperm production, follicular development, ovulation and conception in male and female mammals (2, 3). Vaccination against GnRH
blocks the hypothalamic-pituitary- gonadal axis. 
Therefore, it can be used as an alternative for
castration and fertility control in farm animals,
companion animals and wildlife species (4-6).
Application of GnRH vaccination in humans has
been suggested for controlling fertility-related
endocrine disorders and gonadal steroid-dependent diseases (7). Active immunization of adult
animals against GnRH causes the loss of synthesis
and secretion of gonadotropins and cessation of
gonadal function as long as the antibody titers
* Corresponding Author: 
Vinoy Kumar Shrivastava,
Endocrinology
Laboratory, Department 
of Bioscience, Barkatullah
University, Bhopal (M.P.)
India.
E-mail: 
vinoyks2001@yahoo.com
Received: May 9, 2010
Accepted: Aug. 10, 2010                                      J Reprod Infertil, Vol 12, No 1, Jan/ Mar 2011
JRI Kamdhenu ark and GnRH-BSA immunization
4
remain elevated (8).
It has been reported that cow urine contains all
beneficial elements such as chemical properties,
potentialities and constituents that are capable of
removing all the ill effects and imbalances of
body caused by various infectious agents and
toxicants. In this way, it ensures a protection
against various ailments including the most
dreaded diseases like cancer, diabetes, hepatitis
etc. (9). Kamdhenu ark (distilled cow urine) has
been reported as a strong immunomodulator and
bioenhancer by various researchers (10, 11).
Experimental studies of Rangasamy and Kaliappan revealed the protective effects of cow urine on
haematological, serum biochemical parameters
and immune status of broilers (12). 
The present study attempts to evaluate the
effects of GnRH-BSA immunization on gonadosomatic indices (GSI) and sperm parameters in
male mice and to examine the modulatory role of
Kamdhenu ark following the immunization.
Methods
Sixty adult male mice, Mus musculus, of Parke’s
strain (P), weighing 30±5  g were used in the
study. The animals were divided into three groups
of twenty. The mice in Group I served as the
controls, receiving intraperitoneal Phosphate
Buffered Saline (PBS) injections (100  µl) on the
1
st
, 30
th
, 60
th
 and 90
th
 days, while the mice in
group II were immunized by GnRH-BSA conjugate (50/0.2/35  µg/ml/g BW) (Sigma-Aldrich,
USA) dissolved in 100 µl of phosphate buffered
solution (0.01 N) emulsified with an equal volume
(100  µl) of Freund’s adjuvant (Sigma Aldrich,
USA). GnRH-BSA injections were given intraperitoneally at different intervals, i.e. on days 1
st
 ,
30
th
, 60
th
 and 90
th
 However, the mice in group III .
were supplemented with daily Kamdhenu ark
(100  ppm) (Gaytri Shakti Peeth, India) orally
along with the intraperitoneal injections of GnRHBSA. Five animals from each group were
sacrificed in monthly intervals, i.e. on days 30, 60,
90 and 120 and their testes and epididymides were
quickly dissected. The testes were weighed for
observing gonadosomatic indices [gonad weight/
100  g BW], while the epididymides were processed for semen analysis,  i.e. sperm motility,
sperm count and morphology by Prasad method
(13). Cauda epididymides were dissected out to
release sperms in normal saline (100  mg tissue/ 
2  ml N.S.) for sperm suspension.  For studying
sperm morphology, Leishman`s stain was used
and the slides were finally observed at 400×
magnification (14).
Statistical Analysis: The collected data were analyzed through one way ANOVA and post-hoc
methods using EZANOVA software. P-values
<0.05 or <0.01 were considered significant while
values <0.001 were considered as highly significant.
Results
GSI values decreased in all the experimental
groups compared to the control group. However,
more significant decrease in GSI was observed in
the group treated by Kamdhenu ark along with
GnRH-BSA, especially in the later part of the
experiment (p<0.01) (Table 1). Moreover, sperm
motility and sperm count significantly decreased
throughout the investigation in all the treated
groups compared to the control group (p<0.01)
(Table 2). However, some mice immunized by
GnRH-BSA + Kamdhenu ark also showed decreased values for sperm motility and count than
the GnRH-BSA immunized groups (p<0.05). The
Table 1. Gonadosomatic indices (GSI) in the experimental and control groups of
male mice, Mus musculus,after different intervals
Group
GSI (gonad weight/100 g BW)
Duration
30 days 60 days 90 days 120 days
Control  0.40 ± 0.02  0.43 ± 0.05  0.50 ± 0.01  0.54 ± 0.04
GnRH-BSA   0.36 ± 0.06 0.30  ± 0.02
a
* 0.23 ± 0.00
a
 0.16 ± 0.00
a
GnRH-BSA + KA    0.32±0.03  0.26± 0.01
a
0.19±0.01
ab
0.12±0.00
ab
Mean ± SEM of five animals (Accuracy of calculation up to two decimal digits)
a
 = Significant difference with the controls in the same column (p< 0.01) 
b
= Significant difference with GnRH-BSA groups in the same column (p< 0.01)
* = Significant differences (p< 0.05)                                                                              J Reprod Infertil, Vol 12, No 1, Jan/ Mar 2011
                                                                                            Ganaie JA, et al.  JRI
5
percentage of morphologically normal sperm decreased significantly with increased percentage of
abnormal forms of sperms, i.e. pin head, large
head, oval head, double head, head less, bent
neck, looping mid piece, coiled-tail, double-tailed,
tailless in all the experimental groups as compared
to the control group (p<0.01) (Table 3, Figure 1).
Moreover, some significant alterations in normal
sperm morphology, such as large head, headless
and pin head sperm were also observed in GnRHBSA + Kamdhenu ark treated group when compared with GnRH-BSA, especially in the later part
of the experiment (p<0.01).
Discussion
The endocrine effects of active immunization
against GnRH have been studied in a variety of
young adult male and female animals (15-17).
Experimental studies have demonstrated decreases in gonadotropins, sperm production,
follicular development, ovulation and conception
after immunization against GnRH, chemically
conjugated to a carrier protein. GnRH immuneization affected sperm motility and sperm counts
in ram lambs, boars and colts (18, 19). Several
other experimental studies have revealed the
deleterious effects of immunization against GnRH
on different sperm parameters in rats, bulls,
stallions, cats and dogs (20-24).
Cow urine has been tested for its immunomodulatory properties that enhance both cellular
and humoral immune responses (25, 26). Kamdhenu ark (distilled cow urine) has been reported to
increase the humoral immunity in rats (27). 
Chauhan  et al., (2004) observed that Kamdhenu
ark may modulate the immune responses because
it increases the secretion of interleukin-1 and 2
Table 2. Sperm motility and sperm count in the experimental and control groups of male mice, Mus musculus, after
different intervals
Parameters Group
Duration
30 days 60 days 90 days 120 days
Sperm Motility (%)
Control  59.00±4.33  63.76±2.77  65.05±2.31  69.60±3.19
GnRH-BSA   39.40±3.81
a
 21.56±1.36
 a
 13.00±2.11
 a
 9.40±1.14
 a
GnRH-BSA+ KA  33.80±1.72
 a
17.06±1.00
 ab
* 10.46±1.65
 a
7.16±0.95
 a
Sperm Count (million/ml)
Control  62.00±3.18 68.60±2.10  75.50±3.76 78.00±2.11
GnRH-BSA   49.10±2.65
 a
35.22±3.21
a
21.00±2.20
a
16.42±1.78
a
GnRH-BSA + KA  44.18±2.82
a
 26.12±2.00
 ab
* 18.30±1.90
a
 12.34±1.10
ab
*
Mean ± SEM of five animals (Accuracy of calculation up to two decimal digits)
a
 = Significant difference with the controls in the same column (p< 0.01) 
b
= Significant difference with the GnRH-BSA groups in the same column (p< 0.01)
* = Significant differences (p< 0.05)
Figure 1. Normal morphological sperm forms in the controls
(A) and morphologically abnormal sperms after GnRH-BSA
and Kamdhenu ark along with GnRH-BSA administration (BI) in male Mus musculus.                                      J Reprod Infertil, Vol 12, No 1, Jan/ Mar 2011
JRI Kamdhenu ark and GnRH-BSA immunization
6
(28). Recently, Ganaie and Shrivastava reported
the modulatory effects of Kamdhenu ark on
GnRH-BSA immunization in female mice (29). 
In corroboration to above studies, our study also
revealed that GnRH-BSA immunization significantly decreased the values of GSI, sperm motility, count and morphology in male Mus musculus.
The aforesaid parameters diminished more significantly in the group supplemented with Kamdhenu
ark along with GnRH-BSA immunization. All
these changes in GSI and sperm parameters
suggested that GnRH-BSA immunization could
have directly suppressed the activities of gonadotropins and testosterone through hypothalamohypophysial-gonadal axis or might have indirectly
affected the testicular tissue. However, more
significant decreases in the parameters after
Kamdhenu ark supplementation may be because
of its modulatory and bioenhancing properties.
Acknowledgement
The authors gratefully acknowledge the help and
financial support of Professor Meenakshi Benerjee, the head of Department of Biosciences,
Barkatullah University, Bhopal (M.P), India.
References
1. Garner LL, Campbell GT, Blake CA. Luteinizing
hormone (LH)-releasing hormone: chronic effects
on LH and follicle-stimulating hormone cells and
secretion in adult male rats. Endocrinology. 1990;
126(2):992-100.
2. Hoskinson RM, Rigby RD, Mattner PE, Huynh VL,
D'Occhio M, Neish A, et al. Vaxstrate: an anti reproductive vaccine for cattle. Aust J Biotechnol.
1990;4(3):166-70, 176.
3. Prendiville DJ, Enright WJ, Crowe MA, Vaughan L,
Roche JF. Immunization of prepubertal beef heifers
against gonadotropin-releasing hormone: immune,
estrus, ovarian, and growth responses. J Anim Sci.
1995;73(10):3030-7.
4. Bonneau M, Enright WJ. Immunocastration in cattle
and pigs. Livest Prod Sci. 1995;42(2-3):193-200.
5. Robbins SC, Jelinski MD, Stotish RL. Assessment
of the immunological and biological efficacy of two
different doses of a recombinant GnRH vaccine in
domestic male and female cats (Felis catus). J
Reprod Immunol. 2004;64(1-2):107-19.
6. Miller LA, Johns BE, Killian GJ. Immunocontraception of white-tailed deer with GnRH vaccine. Am J
Reprod Immunol. 2000;44(5):266-74.
Table 3. Percentage of normal and abnormal sperm morphology in the experimental and control groups of male mice, Mus musculus, after different intervals
Days Groups
Normal
 (%)
Abnormal (%)
Pin head
Large
head
Oval
head
Double
head
Head less Bent neck
Looping
mid piece
Coiled tail
Double
tailed
Tail-less
30 
Control  58.44±2.12 5.0±0.35 1.0±0.35 4.0±0.79 1.2±0.65 4.0±1.17 3.0±0.86 4.4±1.60 1.6±0.30 0.0±0.0 2.5±1.00
GnRHBSA
27.77±0.78
 a
8.4±1.03  5.0±0.79  5.80±1.29  2.2±0.74  5.8±1.08  6.4±1.03  6.2±0.65  2.4±0.75  2.2±0.96  5.4±1.15
GnRHBSA+ KA
24.00±1.22
 ab*
 7.6±0.57 4.20±0.65 6.4±1.03 2.4±0.75 8.6±0.90 5.6±1.3 6.5±2.19 1.80±0.24 1.66±0.51 2.5±0.75
60 
Control  64.00±1.41 5.4±0.57 4.2±0.65 3.4±0.57 0.60±0.00 5.0±1.36 3.4±0.57 4.2±0.65 2.5±0.55 1.33±0.51 0.80±0.14
GnRHBSA
23.20±1.16 a  11.2±0.65  10.20±1.74  7.20±0.50  2.60±0.90  7.80±0.65  9.4±1.15  10.80±1.38  4.0±0.70  2.00±0.00  3.5±0.87
GnRHBSA+ KA
20.65±0.94 a 10.80±0.45 6.4±0.59 7.4±0.90 0.00±0.00 11.80±1.43 10.00±1.76 9.60±0.51 2.50±0.48 3.0±1.11 5.80±2.38
90 
Control  67.10±1.74 4.5±0.65 2.60±1.03 2.2±0.41 0.00±0.00 3.401.71 2.60±0.90 1.60±0.83 0.00±0.00 2.60±1.03 1.0±0.21
GnRHBSA
14.24±0.48 a  13.60±1.15  5.80±0.93  6.0±1.00  1.20±0.65  10.40±0.75  12.0±0.79  17.80±1.94  2.50±1.15  1.0±0.25  8.0±0.65
GnRHBSA+ KA
12.72±0.23 ab 13.20±1.19 8.80±0.96 5.80±1.55 2.2±0.96 13.40±1.03 9.5±2.07 10.80±1.29 3.0±0.79 2.5±0.83 11.0±1.76
120 
Control  72.00±1.66 2.60±0.41 2.20±0.39 1.0±0.50 0.00±0.00 2.80±1.29 1.20±0.54 2.0±0.44 1.00±0.00 0.00±0.00 2.5±1.15
GnRHBSA
10.33±0.25
 a
12.80±1.55  7.00±0.79  6.00±0.79  2.80±0.96  16.00±1.36  17.20±0.96  20.60±1.95  5.00±1.11  2.80±0.41  9.50±1.68
GnRHBSA+ KA
7.95±0.40
 ab
 14.60±1.03 11.00±0.79 7.20±0.82 3.80±0.96 17.60±1.07 15.00±1.83 14.25±2.21 4.60±1.15 4.0±1.17 12.50±0.65
Mean ± SEM of five animals (Accuracy of calculation up to two decimal digits)
a = Significant difference with the controls in the same column (p< 0.01) 
b = Significant difference with GnRH-BSA groups in the same column (p< 0.01)
* = Significant differences (p< 0.05)                                                                               J Reprod Infertil, Vol 12, No 1, Jan/ Mar 2011
                                                                                            Ganaie JA, et al.  JRI
7
7. Simms MS, Scholfield DP, Jacobs E, Michaeli D,
Broome P, Humphreys JE, et al. Anti-GnRH antibodies can induce castrate levels of testosterone in
patients with advanced prostate cancer. Br J Cancer.
2000;83(4):443-6.
8. Kumar N, Savage T, DeJesus W, Tsong YY, Didolkar A, Sundaram K. Chronic toxicity and reversibility of antifertility effect of immunization against
gonadotropin-releasing hormone in male rats and
rabbits. Toxicol Sci. 2000;53(1):92-9.
9. Bhadauria  H.  Gomutra-Ek  Chamatkari  Aushadhi
(Cow urine- A Magical therapy). Vish Ayur Patrika.
2002;5:71-4.
10. Chauhan RS, Singh BP, Singhal LK. Immunomodulation with kamdhenu ark in mice. J Immunol
Immunopathol. 2001;3(1):74-7.
11. Garg N, Chauhan RS, Kumar A. Assessing the
effect of cow urine on immunity of white leghorn
layers. International Society for Animal Hugges
(ISAH). 2005;2:81-3.
12. Mathivanan R, Kalaiarasi K. Panchagavya and
Andrographis paniculata as alternatives to antibiotic growth promoters on haematological, serum
biochemical parameters and immune status of
broilers. J Poult Sci. 2007;44(2):198-204.
13. Prasad MR, Chinoy NJ, Kadam KM. Changes in
succinic dehydrogenase levels in the rat epididymis
under normal and altered  physiologic conditions.
Fertil Steril. 1972;23(3):186-90.
14. Highland HN, Rao MV, Chinoy NJ, Shah VC. Analysis of the functional and nuclear integrity of
human spermatozoa. Int J Fertil. 1991;36(1):43-7.
15. Esbenshade KL, Britt JH. Active immunization of
gilts against gonadotropin-releasing hormone: effects on secretion of gonadotropins, reproductive
function, and responses to agonists of gonadotropin-releasing hormone. Biol Reprod. 1985;33
(3):569-77.
16. Johnson HE, DeAvila DM, Chang CF, Reeves JJ.
Active immunization of heifers against luteinizing
hormone-releasing hormone, human chorionic
gonadotropin and bovine luteinizing hormone. J
Anim Sci. 1988;66(3):719-26.
17. Meloen RH, Turkstra JA, Lankhof H, Puijk WC,
Schaaper WM, Dijkstra G, et al. Efficient immunecastration of male piglets by immunoneutralization
of GnRH using a new GnRH-like peptide. Vaccine.
1994;12(8):741-6.
18. Grizzle TB, Esbenshade KL, Johnson BH. Active
immunization of boars against gonadotrop in releasing hormone. I. Effects on reproductive parameters. Theriogenology. 1987;27(4):571-80.
19. Dowsett KF, Pattie WA, Knott LM, Jackson AE,
Hoskinson RM, Rigby RP, et al. A preliminary
study of immunological castration in colts. J
Reprod Fertil Suppl. 1991;44:183-90.
20. McLachlan RI, Wreford NG, Tsonis C, De Kretser
DM, Robertson DM. Testosterone effects on
spermatogenesis in the gonadotropin-releasing hormone-immunized rat. Biol Reprod. 1994;50(2):
271-80.
21. Cook RB, Popp JD, Kastelic JP, Robbins S, Harland R. The effects of active immunization against
gnRH on testicular development, feedlot performance, and carcass characteristics of beef bulls. J
Anim Sci. 2000;78(11):2778-83.
22. Janett F, Stump R, Burger D, Thun R. Suppression
of testicular function and sexual behavior by vaccination against GnRH (Equity) in the adult stallion. Anim Reprod Sci. 2009;115(1-4):88-102.
23. Levy JK, Miller LA, Cynda Crawford P, Ritchey
JW, Ross MK, Fagerstone KA. GnRH immunecontraception of male cats. Theriogenology. 2004;
62(6):1116-30.
24. Ross MK, Miller LA, Crawford PC, Ritchey JW,
Fagerstone KA. GnRH  immunocontraception in
cats. In: Baker H, Boyle S, Griffin B, editors. Proceedings of the 2004 ACCD International Symposium on Non-surgical Methods for Pet Population
Control; 2004 June 24-27; Denver, Colorado, Belle
Court (Portland): ACCD Press; 2005. p.113-5. 
25. Prabhakar K, Singh GK, Chauhan RS, Singh DD.
Effect of cow urine on lymphocyte proliferation in
developing stages of chicks. Indian Cow. 2004;1
(2):3-5.
26. Kumar R, Chauhan RS, Singhal LK, Singh AK,
Singh DD. A comparative study on immunostimulatory effects of Kamdhenu Ark and Vasant Kusumakar in mice. J Immunol Immunopathol. 2002;4
(1-2):104-6.
27. Garg N, Chauhan RS. Kamdhenu ark changes
humoral immunity in rat. In: National Symposium
on Molecular Biology in India- A Post Graduate
Update; 2003 Jan 18, Gwalior, India, Gwalior
(Madhya Pradesh): Cancer Hospital and Research
Institute; 2003. p. 98.
28. Chauhan RS, Singh DD, Singhal LK, Kumar R.
Effect of cow urine on interleukin-1 and 2. J
Immunol Immunopathol. 2004;6(1):38-9.
29. Ganaie JA, Shrivastava VK. Effects of gonadotropin releasing hormone conjugate immunization
and bioenhancing role of Kamdhenu ark on estrous
cycle serum estradiol and progesterone levels in
female Mus musculus. Iran J Reprod Med. 2010;8
(2):70-5.